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Insulator insulation effect formation
- Jun 12, 2018 -

Some enhancers are located upstream of the promoter and some are downstream, so the effect of the insulator does not depend on the relative position of the insulator and the promoter. Therefore, the reason for the directionality of the insulator effect has not been fully understood. Two loci were found to affect the function of the insulator in a transactivation manner. The nuclear protein encoded by the gene S2J (Hw) recognizes the insulator, and the insulator only has the insulation effect after being combined with it. When the gene was mutated, although the insulator was inserted in the y locus, the insulation was lost and y was expressed in all tissues. The other locus is mod (mdg 4). After the gene is mutated, the effect is exactly the opposite of that of Su (Hw). That is, these mutants enhance the insulation and extend the insulating effect of insulators without directionality. That is, it blocks the effects of enhancers on both the upstream and downstream sides. There is an explanation that Su(Hw) is first combined with insulator DNA to make the insulator have an insulating effect. Mod (mdg4) is combined with Su (Hw) to make the insulator lose its insulating effect; the mutated mod (mdg4) cannot be combined with Su (Hw), so the insulator enhances the insulation.